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半抑制浓度IC50

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半抑制浓度(或称半抑制率),即IC50。在间接竞争ELISA标准曲线中是一个非常重要的数据, 标准曲线是一个S型曲线。      ICELISA中,不添加抑制物质的对照孔的OD值为B0, 添加了抑制物质的孔的OD值为B      B/B0% 就叫做结合率, 在结合率为50%时所对应的抑制物质的浓度就叫做IC50.      一般IC50的数值越小,说明你的抗体的特异性能越强!      最小检测限为试剂盒标准曲线的最小的浓度,小于最小浓度或大于最大浓度,即不在曲线范围内的都不准确,大于最大浓度可以稀释后测。      IC50为50%抑制浓度即B/B0=50%时所对应的浓度,半数抑制是用来衡量抗体灵敏度的半数抑制越低,说明抗体的灵敏度越高。      通常来说, 试剂盒最大和最小量程应该是该抗体用来检测标准品的检测限,检测限分最大和最小检测限。      检测下限一般为理论值,是根据标准曲线算出来的理论上可以检测到的最小的值;      定量限为实际检测时可以定量的最低的值;      而最大检测限就是实际操作中抑制率达到100%时的药物浓度。      检测下限(LOD)对应的OD值:OD(LOD) =B0-3SD      定量限(LOQ)对应的OD值:OD(LOQ) = B0-10SD      IC50 wiki的定义:      IC50, the half maximal inhibitory concentration, represents the concentration of an inhibitor that is required for 50% inhibition of things like an enzyme, a cell, a cell receptor or a microorganism.      It's commonly used as a measure of drug effectiveness. Another measure of drug efficacy is EC50 which represents the concentration of a compound that is required to obtain 50% of the maximum effect. (Ex. EC100 would be the minimum concentration of a compound to obtain 100% of its effect).


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